Following transfection, however, both the sense and antisense probes would be expected to recognize vector DNA.

Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules.

Typically, this is done by cleaving the vector DNA and foreign DNA with the same restriction enzyme, for example EcoRI.

Next, the vector DNA can be taken up by a host organism- commonly a population of Escherichia coli or yeast- with each cell containing only one vector molecule.

These fragments are then joined with vector DNA to produce recombinant DNA molecules.

This prevents recircularization of linearized vector DNA and improves the yield of vector containing the appropriate insert.

Therefore, the long DNA molecules that compose an organism's genome must be cleaved into fragments that can be inserted into the vector DNA.

The linear vector DNA already has the topoisomerase enzyme covalently attached to both of its strands' free 3' ends.

The concept is important in molecular biology, especially in cloning or when subcloning inserts DNA into vector DNA.

An expression cassette is a part of a vector DNA used for cloning and transformation.