Each oligonucleotide is designed to be either part of the top or bottom strand of the target sequence.

A primer is added to the sample to identify the target sequence.

Set a lower value if you need to find target sequences with more mismatches to your primers.

Oligonucleotides are designed that are similar to the target sequence.

This doubles the number of potentially useful insertion sites within a given target sequence.

Each of the enzymes has a different target sequence.

This year, the designs of the "target" sequences are changed.

But those target sequences extend for several bases on either side of the cut.

Half of these are "mismatch spots", which do not precisely match the target sequence.

In the presence of the target sequence, the primers match with it and trigger a chain reaction.