These perform only the size separation and peak reading; the actual sequencing reaction(s), cleanup and resuspension in a suitable buffer must be performed separately.

Simple n-butanol purification of dye terminator sequencing reactions.

This DNA was directly used in subsequent sequencing reactions.

So, for a sequencing reaction, researchers use a mix of nucleotides where the majority are normal but a small fraction lack the hydroxyl group.

This method is now used for the vast majority of sequencing reactions as it is both simpler and cheaper.

The chemistry of the 454 sequencing reaction is radically different, and goes by the catchy name pyrosequencing.

The composition of bases n+3,n+4 and n+5 remains undetermined until further rounds of the sequencing reaction.

The basic procedure in an Illumina dye sequencing reaction is as follows.

Maxam-Gilbert sequencing reactions were performed as described [ 37 ] .

The products of displaced strands can be subsequently cloned into vectors to construct library for subsequent sequencing reactions.