Finally, unanchored ESTs and gene clusters of size 1 (which may represent rare transcripts) are compared with other UniGene clusters at lower stringency.

The third and fourth categories consist of clones that may represent alternative transcripts (138) and clones that are currently computationally unclassified (417), respectively.

As the ethanol control treatment did not appear to affect gene expression significantly, we analyzed another similar R1881 time course in which cDNAs representing transcripts from treated (Cy-5 labeled) and control (Cy-3 labeled) cells were compared directly by hybridization to the same microarrays.

Sequence data We obtained sequence data for 271 independently isolated cDNAs representing transcripts expressed in the Drosophila brain (Figure 1, Table 2).

The remaining 5,532 DTs with mRNA sequences might represent alternative transcripts of MGI genes, known genes not yet represented by MGI, or novel genes.

Contig member ESTs should all derive from identical transcripts whereas cluster members might derive from the same gene yet represent different transcript splice isoforms or transcripts from multigene families with extremely high sequence identity.

Of the 56 most abundantly expressed tags in NGF-treated cells, 21 represent ribosomal transcripts.

We were also interested in whether sense and antisense RNA species in these candidate clusters represented protein-coding or untranslated transcripts.

Alternatively, since the Brugia ESTs derive from 12 different libraries they may represent rarer transcripts than are contained in the M. incognita collection.

It is possible that they represent transcripts in which exon 1b is spliced to exons other than 1b'.