This leads to a probe sequence of where the values increase by 1, 2, 3, ...

The oligonucleotide is designed such that there are complementary regions at each end and a probe sequence located in between.

The mixture of probe sequences determines the type of feature the probe can detect.

Furthermore, existing probe sets are revised because probe sequences once thought to be unique for a single gene are occasionally found to be less specific.

Absolute values for the same transcript concentration differ significantly, despite averaging over 20 probe pairs, which shows the influence of selected probe sequences (Table 1).

However, in one probe sequence (Figure 4C), specificity was enhanced.

Full substitution of adenosine by DAP showed varied effects on specificity in the four different probe sequences.

In addition the quality of the probe sequences and the location of the probes selected for incorporation into the array are also very important.

Relevant probe sequences for small subunit RNA can be found in Bowers et al. 2006.

Well known probe sequences include: