Inaccuracies in the Release 1 and 2 predicted gene structures resulted mainly from computationally predicted annotations which lacked supporting cDNA data.

However, structures of many membrane-associated proteins are not included in the database if they can not be computationally predicted.

There are, however, several private and public databases devoted to compilation of experimentally reported, and sometimes computationally predicted, binding sites for different transcription factors in different organisms.

Lumenal proteins can be predicted computationally based on their targeting signals.

In fact, it has been predicted computationally that the hexazine molecule is highly unstable.

In some of these species the presence of miR-10 has been shown experimentally, in others the genes encoding miR-10 have been predicted computationally.

It is often difficult to determine which functions have been determined experimentally and which are predicted computationally.

Furthermore, for computationally predicted functions, the method used to make the prediction and the strength of the evidence are rarely stated.

Additionally, functions identified from high throughput experiments as well as computationally predicted function annotations are included from GO Annotation project.

Their RNA secondary structures have been predicted computationally, the complementary regions appear to be presented on exposed loops for interaction.