Greenleaf was elected colonel of the regiment October 23, 1862.

Jordan E. Cravens was elected colonel of the new unit.

Ashby was elected colonel of the regiment on May 24, 1862.

According to one participant, he had been elected colonel of his consolidated regiment the day before.

Adams was elected colonel on April 25, 1862.

He was elected colonel and given command of the Confederate provisional forces in eastern Florida.

Later in the same year he was elected colonel of the 2nd Florida Infantry.

That June he was elected colonel, assuming command of the regiment.

In 1867 he was elected colonel, which command he held for more than twenty years.

A year later, he was elected colonel of the regiment.