The capacitor is charged through the resistor, causing the voltage across the capacitor to approach the charging voltage on an Exponential decay curve.

If it's going that fast we'll have to recalculate all emission records on the decay curve.

The goal of the analysis algorithm is to extract the pure decay curve from the measured decay and to estimate the lifetime(s).

There are a few techniques which work in transformed space that exploit this property to recover the pure decay curve from the measured curve.

In Fourier methods the lifetime of a single exponential decay curve is given by:

Decay curves (fluorescence intensity vs. time) are recorded and the area between the two decay curves (with or without antioxidant) is calculated.

Heat transfer experiments yield results whose best fit line are exponential decay curves.

If we have decay curve which is represented by an exponential function with lifetime of τ:

When the dye is attached to a macromolecules the decay curve becomes multiexponential.

Analog has a smooth decay curve, but digital has a jagged steppy one.