This test is about 2 -fold more sensitive than direct sequencing (see Methods; data not shown).

One of the primers was biotinylated to facilitate subsequent direct sequencing.

Direct sequencing was used to ensure in-frame insertions of these constructs.

The alternative splice variants were confirmed by direct sequencing.

Initially the primers were used to prepare amplicons of dystrophin exons for direct sequencing.

No direct sequencing was done to verify the CGH findings in these loci.

Two affected individuals from each of six families with CFEOM1 were analyzed by direct sequencing.

However, this form of direct sequencing destroys the original sample so to obtain new reads more sample must be destructively sequenced.

Mutations in either case are essentially unpredictable and must be mapped by elaborate techniques such as classical complementation analyses or direct sequencing of the genome.

Direct sequencing of PCR products was completed using cycle sequencing.